<b>Differential of expression in Isoenzymas seeds and seedlings F1 of barley</b>

Authors

  • Lilian Madruga Tunes Universidade Federal de Santa Maria
  • Pablo Gerzson Badinelli UFPel
  • Maria Alice da Silva de Castro UFPel
  • Antonio Carlos de Souza Albuquerque Barros UFPel

DOI:

https://doi.org/10.5777/paet.v2i2.88

Keywords:

Hordeum vulgare L., electrophoresis, protein extraction, ACP, ADH and GOT

Abstract

The present work had as objective to evaluate the isoenzimatic patterns of Acid Phosphatase (ACP), Alcohol Dehydrogenase (ADH) and Glutamate Oxalacetate Transaminase (GOT) of two barley cultivars (MN 721 and Scarlett). The seeds were picked in three times with different percentage corresponding to three different steps of their maturation. The seed humidity was reduced to 13% and stored in cold camera. The seeds and the plantlet were analyzed of all cultivars and times. The three isoenzimatic systems analyzed presented variations in the expression, as it was expected when compared between seeds and seedlings. The water content of seeds influences the enzyme pattern of the GOT enzyme. ACP bands were detected in all the treatments evaluated, however, in seeds with lower intensity.

Author Biographies

Lilian Madruga Tunes, Universidade Federal de Santa Maria

Doutoranda do curso de Agronomia do Departamento de Fitotecnia- Centro de Ciências Rurais Universidade Federal de Santa Maria - UFSM

Pablo Gerzson Badinelli, UFPel

Engenheiro Agrônomo. Estudante do Curso de Pós-Graduação em Fisiologia Vegetal – FAEM – UFPel.

Maria Alice da Silva de Castro, UFPel

Técnica em Química. Técnica Laboratório de Bio Sementes / FAEM/UFPel.

Antonio Carlos de Souza Albuquerque Barros, UFPel

Engenheiro Agrônomo, Dr., Professor, Departamento de Fitotecnia, FAEM/UFPel, Caixa Postal: 354, CEP 96001-970, Pelotas-RS

Published

01-05-2011

Issue

Section

Articles